Non-T Cell Activation Linker Promotes Mast Cell Survival by Dampening the Recruitment of SHIP1 by Linker for Activation of T Cells

摘要: The linker for activation of T cells (LAT) and the non-T cell (NTAL) are two transmembrane adapters which organize IgE receptor (FceRI) signaling complexes in mast cells. LAT positively regulates, whereas NTAL negatively regulates activation. We previously found that four distal tyrosines can generate negative signals. show here these provide binding sites SHIP1, recruits SHIP1 vivo, recruitment is enhanced NTAL-deficient by decreasing recruitment, LAT, molecules involved FceRI-dependent positive signaling. also decreases leading to an increased phosphorylation antiapoptotic molecule Akt, survival. finally effect on Akt survival requires LAT. Our data thus document mechanisms both signals differentially regulate They molecular bases FceRI complexes. a major regulator and, hence, allergic reactions.