Comparative Expression Study of the Endo–G Protein Coupled Receptor (GPCR) Repertoire in Human Glioblastoma Cancer Stem-like Cells, U87-MG Cells and Non Malignant Cells of Neural Origin Unveils New Potential Therapeutic Targets

摘要: Glioblastomas (GBMs) are highly aggressive, invasive brain tumors with bad prognosis and unmet medical need. These heterogeneous being constituted by a variety of cells in different states differentiation. Among these, endowed stem properties, tumor initiating/propagating properties particularly resistant to chemo- radiotherapies designed as the real culprits for maintenance relapse after treatment. cells, termed cancer stem-like have been prominent targets new more efficient therapies. G-protein coupled receptors (GPCRs), family membrane receptors, play role cell signaling, communication crosstalk microenvironment. Their has highlighted but remains largely unexplored. Here, we report descriptive study differential expression endo-GPCR repertoire human glioblastoma (GSCs), U-87 MG astrocytes fetal neural (f-NSCs). The transcriptome studied using Taqman Low Density Arrays. Of 356 GPCRs investigated, 138 were retained comparative studies between types. At transcriptomic level, eight specifically expressed/overexpressed GSCs. Seventeen appeared expressed (GSCs f-NSCs). Results GPCR at protein level mass spectrometry proteomic analysis also presented. presented here gives clues pathways used GSCs unveils novel potential therapeutic targets.