Mycoplasma Virus P1 Has a Linear, Double-Stranded DNA Genome with Inverted Terminal Repeats

摘要: Abstract Mycoplasma virus P1 is a tailed, polyhedral isolated from pulmonis . To characterize the genome, stocks of were prepared free host cell nucleic acids. A single DNA species 11.3 kb that was shown by plaque hybridization to be origin extracted virus. Efficient isolation required digestion with proteolytic enzymes prior phenol extraction. Although double-stranded and linear following such treatment, it resistant 5′-specific λ exonuclease. Electron microscopic analysis indicated globular material complexed ends DNA. The not observed on protease-treated molecules, suggesting composed protein. Removal putative terminal protein chemical treatment allowed cloning ends, nucleotide sequence revealed these contain 350-bp inverted repeat.