CHARACTERIZATION OF THE LYSOGENIC BACTERIOPHAGE MAV1 FROM MYCOPLASMA ARTHRITIDIS
作者： LeRoy L. Voelker , Kevin Dybvig
DOI: 10.1128/JB.180.22.5928-5931.1998
关键词:
摘要: The lysogenic bacteriophage MAV1, which is associated with the arthritogenicity of Mycoplasma arthritidis, was characterized. Several strains M. arthritidis were examined for their ability to support growth MAV1. A PFU assay developed, and sensitivity phage various chemical treatments assayed. most notable result resistance MAV1 proteinase K. genome a double-stranded, linear DNA molecule about 16 kb. site integration in host chromosome investigated. ends cloned from three independent lysogens shown have inserted at different sites host. nucleotide sequences DNA-chromosomal junctions each determined. Sequences flanking integrated prophage native determined, allowing identification (attP) bacterial (attB) recombination sites. Analysis left junction showed single-base heterogeneity located within immediately adjacent attB sequence. model integration-excision proposed.
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