Histological approaches for high-quality imaging of zooplanktonic organisms

摘要: The investigation of the internal organization zooplankton communities provides important information on plankton biology with special interest for study ecological processes. Zooplanktoners can play a structural function as indicators ecosystem health or stress, but their using histological techniques is still limited. Here we report that structure zooplanktonic organisms be facilely observed by approach combines optimal fixation and processing plastic resin (glycol methacrylate) embedding, resulting in increased tissue resolution. Using copepods, dominate assemblages, models, collected from tropical (Paraibuna river, Brazil), showed fine details muscular, nervous digestive systems, appendages cell features. Critical advantages this are it permits preservation adequate handling (embedded agar after fixation) further investigation. This because prevents both mechanically induced artifacts loss these diminutive during different steps processing. Moreover, embedding superior imaging copepod structures compared to paraffin embedding. use glycol methacrylate advantageous over paraffin/paraplast avoiding heat damage, retraction allowing faster procedure better value approaches enabling high-quality copepods particularly used environmental changes.