Effects of storage type and time on DNA amplification success in tropical ungulate faeces.
作者： IVÁN D. SOTO-CALDERÓN , STEPHAN NTIE , PATRICK MICKALA , FIONA MAISELS , ELIZABETH J. WICKINGS
DOI: 10.1111/J.1755-0998.2008.02462.X
关键词:
摘要: The present study compares the effect of three storage media (silica, RNAlater®, ethanol) and time to extraction (1 week, 1 month and 3 months) on mitochondrial and nuclear marker amplification success in faecal DNA extracts from a sympatric community of small to medium‐sized Central African forest ungulates (genera Cephalophus, Tragelaphus, Hyemoschus). The effect of storage type and time on nuclear DNA concentrations, genotyping errors and percentage recovery of consensus genotypes was also examined. Regardless of storage …
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