Otter Lutra lutra movements assessed by genotyped spraints in southern Italy

作者: Claudio Prigioni , Luigi Remonti , Alessandro Balestrieri

DOI: 10.4404/HYSTRIX-17.1-4367

关键词: LontraOtterForestryGeographyLutraDemography

摘要: Abstract This paper analyses some otter ( Lutra lutra ) spatial data, obtained by the location of genetically typed spraints in Pollino national park and surrounding areas (southern Italy). A sample 214 was collected (27 faeces 2001-02 187 2004) along 10 streams park, their digitalized a Geographic Information System (Arcview 3.1) for analysis. DNA extracted using GuSCN/silica method each faecal genotyped PCR amplification twelve microsatellite loci. Finally, 103 (48.1%) yielded reliable genotype, providing identification 31 otters. The maximum distance, measured watercourses between samples individual identified at least two faeces, varied from 0.02 to 34.8 km. On whole, our results outlined ranging pattern based on occupation one long stretch watercourse (21.6 km), including 4-6 partially overlapped short stretches (from 14.1 km) occupied as many animals. RIASSUNTO – Spostamenti della Lontra valutati mediante analisi genetica delle feci Italia meridionale. Tramite l’estrazione del da “fresche” e la succes- siva amplificazione di 12 loci microsatellite, un campione raccolto lungo corsi idrici Parco Nazionale aree limitrofe nel e 187 sono stati ottenuti genotipi affidabili (48,1%), consentendo l’identifi- cazione individui. La localizzazione delle genotipizzate stata stabilita mediante GPS (Global Positioning System) trasferita su una mappa digitalizzata (scala 1:10000) tramite GIS (Geographic Information System). distanza massima, misurata lungo i corsi idrici investigati, tra campioni fecali tipizzati ciascun individuo variata 0,02 34,8 km. Nel complesso, risultati hanno evidenziato modello ripartizione spaziale delle lontre consistente nell’occupazione parte tratto corso idrico (21,6 34,8 comprendente 6-9 tratti piu brevi (da 14,1 parte sovrapposti occupati altrettanti

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