Development of novel real‐time TaqMan® PCR assays for the species and sex identification of otter (Lutra lutra) and their application to noninvasive genetic monitoring
作者： David O'Neill , Peter D. Turner , Denise B. O'Meara , Elizabeth A. Chadwick , Lee Coffey
关键词:
摘要: Developing strategies to maintain biodiversity requires baseline information on the current status of each individual species. The development genetic techniques and their application noninvasively collected samples have potential yield structure elusive animal populations so are important tools in conservation management. Using DNA isolated from faecal can be challenging owing low quantity quality. This study, however, presents novel real-time polymerase chain reaction assays using fluorescently labelled TaqMan® MGB probes enabling species sex identification Eurasian otter (Lutra lutra) spraints (faeces). These also used determining an optimum microsatellite panel employed as cost-saving screening for downstream testing including genotyping haplotype analysis. shown work efficiently with L. lutra tissue, hair, spraint, blood anal jelly samples.
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sci-hub.se 参考文章(31)
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