Amplification of hypervariable simple sequence repeats (microsatellites) from excremental DNA of wild living bonobos (Pan paniscus)
作者： U. GERLOFF , C. SCHLÖTTERER , K. RASSMANN , I. RAMBOLD , G. HOHMANN
DOI: 10.1111/J.1365-294X.1995.TB00247.X
关键词:
摘要: We show that nuclear DNA extracted from faeces of free living bonobos (Pan paniscus) can be used to amplify hypervariable simple sequence repeats, which for paternity analysis and kinship studies. Of 130 extractions samples 33 different animals, about two-thirds yielded PCR products at the first attempt. For several only a second extraction resulted in positive amplifications. Consistency tests revealed some cases one two alleles was amplified. Presumably this is due very limited amount bonobo sample we suggest therefore found homozygous given locus should typed repeatedly verification.
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