Amplification success and feasibility of using microsatellite loci amplified from dung to population genetic studies of the Asian elephant (Elephas maximus)
作者： T. N. C. Vidya , R. Sukumar
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摘要: The use of microsatellite DNA markers in large mammal conservation genetics has been limited by the logistic difficulty obtaining optimal sources from free-ranging animals. Here, we show high amplification success elephant dung samples collected under field conditions. Amplification depended on how fresh sample was when collected, and sensitivity primers at individual loci. No significant allelic dropout observed. We also examined loci for selective neutrality, independent inheritance loci, probability identity individuals to confirm their utility studies population genetic structure.
参考文章(21)
Raman Sukumar, The living elephants : evolutionary ecology, behavior, and conservation Oxford University Press. ,(2003)
P. TABERLET, J.-J. CAMARRA, S. GRIFFIN, E. UHRÈS, O. HANOTTE, L. P. WAITS, C. DUBOIS-PAGANON, T. BURKE, J. BOUVET, Noninvasive genetic tracking of the endangered Pyrenean brown bear population. Molecular Ecology. ,vol. 6, pp. 869- 876 ,(1997) , 10.1111/J.1365-294X.1997.TB00141.X
Michael H. Kohn, Eric C. York, Denise A. Kamradt, Gary Haught, Raymond M. Sauvajot, Robert K. Wayne, Estimating population size by genotyping faeces Proceedings of The Royal Society B: Biological Sciences. ,vol. 266, pp. 657- 663 ,(1999) , 10.1098/RSPB.1999.0686
David Paetkau, Lisette P. Waits, Peter L. Clarkson, Lance Craighead, Ernie Vyse, Ryk Ward, Curtis Strobeck, Variation in Genetic Diversity across the Range of North American Brown Bears Conservation Biology. ,vol. 12, pp. 418- 429 ,(1998) , 10.1111/J.1523-1739.1998.96457.X
J. J Constable, C. Packer, D. A Collins, A. E Pusey, Nuclear DNA from primate dung Nature. ,vol. 373, pp. 393- 393 ,(1995) , 10.1038/373393A0
Pierre Taberlet, Lisette P Waits, Gordon Luikart, None, Noninvasive genetic sampling: look before you leap Trends in Ecology and Evolution. ,vol. 14, pp. 323- 327 ,(1999) , 10.1016/S0169-5347(99)01637-7
Lori S. Eggert, Caylor A. Rasner, David S. Woodruff, The evolution and phylogeography of the African elephant inferred from mitochondrial DNA sequence and nuclear microsatellite markers. Proceedings of The Royal Society B: Biological Sciences. ,vol. 269, pp. 1993- 2006 ,(2002) , 10.1098/RSPB.2002.2070
Francisco Palomares, José A Godoy, Ana Píriz, Stephen J O'Brien, Warren E Johnson, Faecal genetic analysis to determine the presence and distribution of elusive carnivores: design and feasibility for the Iberian lynx. Molecular Ecology. ,vol. 11, pp. 2171- 2182 ,(2002) , 10.1046/J.1365-294X.2002.01608.X
Prithiviraj Fernando, T. N. C. Vidya, Don J. Melnick, Isolation and characterization of tri‐ and tetranucleotide microsatellite loci in the Asian elephant, Elephas maximus Molecular Ecology Notes. ,vol. 1, pp. 232- 233 ,(2001) , 10.1046/J.1471-8278.2001.00082.X
Lisette P Waits, Gordon Luikart, Pierre Taberlet, None, Estimating the probability of identity among genotypes in natural populations: cautions and guidelines Molecular Ecology. ,vol. 10, pp. 249- 256 ,(2001) , 10.1046/J.1365-294X.2001.01185.X