Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA
作者： Alida de Flamingh , Catherine L. Sole , Rudi J. van Aarde
DOI: 10.1007/S12686-014-0160-5
关键词:
摘要: Degradation reduces DNA quality and quantity in faecal samples leads to low amplification success. We investigated the influence of repeat motif amplicon length by comparing success five dinucleotide, tetranucleotide, two compound microsatellite markers for African elephant (Loxodonta africana) samples. found that both influenced success, suggest use simple containing alleles with small sizes maximise degraded DNA.
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T. N. C. Vidya, R. Sukumar, Amplification success and feasibility of using microsatellite loci amplified from dung to population genetic studies of the Asian elephant (Elephas maximus) Current Science. ,vol. 88, pp. 489- 492 ,(2005)
Simone Lampa, Bernd Gruber, Klaus Henle, Marion Hoehn, An optimisation approach to increase DNA amplification success of otter faeces Conservation Genetics. ,vol. 9, pp. 201- 210 ,(2008) , 10.1007/S10592-007-9328-9
Maxine P. Piggott, Eva Bellemain, Pierre Taberlet, Andrea C. Taylor, A Multiplex Pre-Amplification Method that Significantly Improves Microsatellite Amplification and Error Rates for Faecal DNA in Limiting Conditions Conservation Genetics. ,vol. 5, pp. 417- 420 ,(2004) , 10.1023/B:COGE.0000031138.67958.44
Yasuko Ishida, Yirmed Demeke, Peter J. van Coeverden de Groot, Nicholas J. Georgiadis, Keith E. A. Leggett, Virginia E. Fox, Alfred L. Roca, Short amplicon microsatellite markers for low quality elephant DNA Conservation Genetics Resources. ,vol. 4, pp. 491- 494 ,(2012) , 10.1007/S12686-011-9582-5
Thomas Broquet, Nelly Ménard, Eric Petit, Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates Conservation Genetics. ,vol. 8, pp. 249- 260 ,(2006) , 10.1007/S10592-006-9146-5
Cock Van Oosterhout, William F Hutchinson, Derek PM Wills, Peter Shipley, None, Micro-Checker: Software for identifying and correcting genotyping errors in microsatellite data Molecular Ecology Notes. ,vol. 4, pp. 535- 538 ,(2004) , 10.1111/J.1471-8286.2004.00684.X
L. S. Eggert, U. Ramakrishnan, N. I. Mundy, D. S. Woodruff, Polymorphic microsatellite DNA markers in the African elephant (Loxondonta africana) and their use in the Asian elephant (Elephas maximus). Molecular Ecology. ,vol. 9, pp. 2222- 2224 ,(2000) , 10.1046/J.1365-294X.2000.105336.X
Melanie A. Murphy, Katherine C. Kendall, Andrew Robinson, Lisette P. Waits, The impact of time and field conditions on brown bear (Ursus arctos) faecal DNA amplification Conservation Genetics. ,vol. 8, pp. 1219- 1224 ,(2007) , 10.1007/S10592-006-9264-0
E. A. Archie, C. J. Moss, S. C. Alberts, Characterization of tetranucleotide microsatellite loci in the African Savannah Elephant (Loxodonta africana africana) Molecular Ecology Notes. ,vol. 3, pp. 244- 246 ,(2003) , 10.1046/J.1471-8286.2003.00412.X
Kenine E. Comstock, Samuel K. Wasser, Elaine A. Ostrander, Polymorphic microsatellite DNA loci identified in the African elephant (Loxodonta africana) Molecular Ecology. ,vol. 9, pp. 1004- 1006 ,(2000) , 10.1046/J.1365-294X.2000.00939-8.X