Genome-wide investigation and transcriptional analysis of cytosine-5 DNA methyltransferase and DNA demethylase gene families in tea plant (Camellia sinensis) under abiotic stress and withering processing.

摘要: DNA methylation is a highly conserved epigenetic modification involved in many biological processes, including growth and development, stress response, secondary metabolism. In the plant kingdom, cytosine-5 methyltransferase (C5-MTase) demethylase (dMTase) genes have been identified some species. However, to best of our knowledge, no investigator has focused on identification analysis C5-MTase dMTase tea plants (Camellia sinensis) based genome-wide levels. this study, eight CsC5-MTases four dMTases were plants. These CsC5-MTase divided into subfamilies, CsMET, CsCMT, CsDRM CsDNMT2. The CsdMTase can be classified CsROS, CsDME CsDML. Based domain these genes, gene loss duplication events occurred during evolution CsdMTase. Furthermore, multiple cis-acting elements observed CsdMTase, light responsiveness, phytohormone development-related elements. Then, we investigated transcript abundance under abiotic (cold drought) withering processing (white oolong tea). Notably, most CsC5-MTases, except for CsCMT1 CsCMT2, significantly downregulated stress, while all was induced. Similarly, same found white tea, respectively. total, findings will provide basis roles response potential functions two families affecting flavor processing.