Transformation Proteins and DNA Uptake Localize to the Cell Poles in Bacillus subtilis
作者： Jeanette Hahn , Berenike Maier , Bert Jan Haijema , Michael Sheetz , David Dubnau
DOI: 10.1016/J.CELL.2005.04.035
关键词:
摘要: The Gram-positive, rod-forming bacterium Bacillus subtilis efficiently binds and internalizes transforming DNA. localization of several competence proteins, required for DNA uptake, has been studied using fluorescence microscopy. At least three proteins (ComGA, ComFA, YwpH) are preferentially associated with the cell poles appear to colocalize. This association is dynamic; accumulate at as transformability develops then delocalize wanes. binding uptake also occur poles, shown fluorescent in single-molecule experiments laser tweezers. In addition prominent polar sites, localize foci lateral membrane, but this distribution does not exhibit same temporal changes accumulation. results suggest regulated assembly disassembly a DNA-uptake machine poles.
unirioja.es
core.ac.uk
elsevier.com
sci-hub.se 参考文章(44)
David Dubnau, Rosa Davidoff-Abelson, Fate of transforming DNA following uptake by competent Bacillus subtilis Journal of Molecular Biology. ,vol. 56, pp. 209- 221 ,(1971) , 10.1016/0022-2836(71)90460-8
David Dubnau, Charles M. Lovett, Transformation and Recombination Bacillus subtilis and Its Closest Relatives. pp. 453- 471 ,(2002) , 10.1128/9781555817992.CH32
B.-J. Haijema, J. Hahn, J. Haynes, D. Dubnau, A ComGA-dependent checkpoint limits growth during the escape from competence. Molecular Microbiology. ,vol. 40, pp. 52- 64 ,(2001) , 10.1046/J.1365-2958.2001.02363.X
Yuh Nung Jan, Lily Yeh Jan, Asymmetric cell division Nature. ,vol. 392, pp. 775- 778 ,(1998) , 10.1038/33854
R. N. Singh, Number of Deoxyribonucleic Acid Uptake Sites in Competent Cells of Bacillus subtilis Journal of Bacteriology. ,vol. 110, pp. 266- 272 ,(1972) , 10.1128/JB.110.1.266-272.1972
David Dubnau, Carol Cirigliano, Fate of transforming DNA following uptake by competent Bacillus subtilis: IV. The endwise attachment and uptake of transforming DNA☆ Journal of Molecular Biology. ,vol. 64, pp. 31- 46 ,(1972) , 10.1016/0022-2836(72)90319-1
Mathieu Bergé, Isabelle Mortier-Barrière, Bernard Martin, Jean-Pierre Claverys, Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands. Molecular Microbiology. ,vol. 50, pp. 527- 536 ,(2003) , 10.1046/J.1365-2958.2003.03702.X
Randy M. Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau, Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK. Molecular Microbiology. ,vol. 43, pp. 1331- 1345 ,(2002) , 10.1046/J.1365-2958.2002.02833.X
Petra Anne Levin, 6 Light microscopy techniques for bacterial cell biology Methods in Microbiology. ,vol. 31, pp. 115- 132 ,(2002) , 10.1016/S0580-9517(02)31007-9
Jeffery Errington, Dirk-Jan Scheffers, Laura J. F. Jones, Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis. Molecular Microbiology. ,vol. 51, pp. 749- 764 ,(2003) , 10.1046/J.1365-2958.2003.03854.X