Number of Deoxyribonucleic Acid Uptake Sites in Competent Cells of Bacillus subtilis
作者： R. N. Singh
DOI: 10.1128/JB.110.1.266-272.1972
关键词:
摘要: Two direct methods are presented for estimating the average number of deoxyribonucleic acid (DNA) uptake sites in competent cells Bacillus subtilis from measurement (14)C- or (3)H-thymine-labeled DNA by culture. Advantage is taken two facts: (i) effective contact between and transforming molecules established within a short time after mixing them together, (ii) enter B. linear fashion at finite speed. From initially attached to brief exposure first method rate culture second method, calculated be 20 53 per cell.
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