Mechanism of Bacterial Transformation and Transfection

摘要: Publisher Summary This chapter discusses that in spite of the large amount information on physiology competent cell, including production competence factors a number transformation systems and an additional protein must be synthesized to permit irreversible binding DNA by D. pneurnoniae, mechanism uptake remains obscure. process requires energy, divalent cations, results breakage incoming DNA. The apparently enters lengthwise, case one phage DNA, particular end first. describes enzymology recombination transfection is difficult problem solve, as there are indications alternative enzymatic pathways for recombination, so mutants blocked pathway may not necessarily show loss ability transformed or transfected. There also unpleasant possibility cells with deficiencies more than inviable, making it assign biological roles enzymes. enzymological studies systems, together intermediates by-products involvement membranes will provide coherent picture during next few years.