Fractionation of Transformable Bacteria from Competent Cultures of Bacillus subtilis on Renografin Gradients

作者: Florence Haseltine Cahn , Maurice S. Fox

DOI: 10.1128/JB.95.3.867-875.1968

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摘要: A population of Bacillus subtilis can be fractionated into at least two components on the basis their buoyant densities a Renografin gradient. In competent cultures, most, and perhaps all, bacteria appear in lighter fraction which composes 2 to 10% bulk population. These “lighter” are only capable incorporating transforming deoxyribonucleic acid. Images

参考文章(15)
Joshua Lederberg, Isolation and Characterization of Biochemical Mutants of Bacteria Lederberg, Joshua. "Isolation and Characterization of Biochemical Mutants of Bacteria." Volume 3 in Methods of Medical#N# Research, edited by Ralph W. Gerard. Yearbook Medical Publishers, Inc., 1950. Pp. 5-22. Excerpt. Monograph. 19 Images.. ,(1950)
Hadassah Tamir, Charles Gilvarg, Density Gradient Centrifugation for the Separation of Sporulating Forms of Bacteria Journal of Biological Chemistry. ,vol. 241, pp. 1085- 1090 ,(1966) , 10.1016/S0021-9258(18)96806-7
E. W. Nester, B. A. D. Stocker, BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACID TRANSFORMATION IN BACILLUS SUBTILIS Journal of Bacteriology. ,vol. 86, pp. 785- 796 ,(1963) , 10.1128/JB.86.4.785-796.1963
R. N. SINGH, M. P. PITALE, Enrichment of Bacillus subtilis Transformants by Zonal Centrifugation Nature. ,vol. 213, pp. 1262- 1263 ,(1967) , 10.1038/2131262A0
Sol H. Goodgal, Roger M. Herriott, Studies on transformations of Hemophilus influenzae. I. Competence. The Journal of General Physiology. ,vol. 44, pp. 1201- 1227 ,(1961) , 10.1085/JGP.44.6.1201
R. D. Hotchkiss, J. Marmur, DOUBLE MARKER TRANSFORMATIONS AS EVIDENCE OF LINKED FACTORS IN DESOXYRIBONUCLEATE TRANSFORMING AGENTS. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 40, pp. 55- 60 ,(1954) , 10.1073/PNAS.40.2.55