T cell gelatinases mediate basement membrane transmigration in vitro.

摘要: T cell homing into extravascular sites requires penetration across the subendothelial basal lamina, a specialized nonfibrillar connective tissue structure that anchors endothelial cells to parenchymal surfaces. Herein, we show normal human express gelatinases A and B, two matrix metalloproteinases active against major lamina constituents, collagen types IV V. Expression is confirmed at both mRNA protein levels. Gelatinase B expressed constitutively, whereas expression induced by activation. In vitro migration of resting equivalent mediated gelatinase because it specifically blocked GM6001, hydroxamic acid inhibitor metalloproteinases. Inhibition interference with function may represent useful approach treatment cell-mediated autoimmune diseases.