Changing pattern of neonatal bacteremia. Microbiology and antibiotic resistance.

摘要: OBJECTIVES To determine the most important causative agents of bacteremia in a neonatal intensive care unit (NICU); their changing distribution and antibiotic susceptibility patterns over 5-year period. METHODS This study was performed between January 1997 2001 at Microbiology Section, Tripoli Medical Center, Tripoli, Libya. During this period 1431 Oxoid Signal Blood Cultures sets were obtained from 1092 NICU with suspected bacteremia. Conventional methods, API 20 E NE used to identify isolated bacteria. The Kirby-Bauer disk diffusion method assess susceptibilities accordance National Committee for Clinical Laboratory Standards. RESULTS During period, 801 out total blood cultures positive microbial growth, which represented 648 cases cases. From number isolates, members Enterobacteriaceae Serratia, Klebsiella Enterobacter spp common cause coagulase negative Staphylococci also frequently isolated. A pattern pathogen observed during 3 leading genera Enterobacteriaceae. Antibiotic testing showed high level resistance among pathogens. Resistance aztreonam, imipenem, ciprofloxacin piperacillin/tazobactam less encountered. Staphylococcus anti-staphylococcal due hyperproduction penicillinase enzyme all isolates remained sensitive vancomycin. CONCLUSIONS Gram-negative bacteria especially are causes NICUs. Although remain new antibiotics, emergence resistant strains cannot be excluded future. For that reason, strategies continuous surveillance required monitor epidemiology pathogens, use needed overcome increasing incidence conventional drugs.