Survival of a novel endophytic fungus Phomopsis liquidambari B3 in the indole-contaminated soil detected by real-time PCR and its effects on the indigenous microbial community.

摘要: Abstract The recently isolated fungal strain Phomopsis liquidambari B3 can degrade high concentrations of indole, indicating its potential for the bioremediation indole-contaminated soil. In this study, a specific real-time PCR was developed to detect survival P. in Subsequently, degradation activity and effects on indigenous microbial community were analyzed. Results showed amount genomic DNA increased maximum 5.67 log (pg g −1 dry soil) 10 days after inoculation 5.04 soil), then gradually decreased with time 40 it below detection limit. By end experiment (day 40), bioaugmented microsoms 93.7% decrease while values biostimulated control microcosms much lower. Higher biomass enzyme activities observed Denaturing gradient gel electrophoresis analysis bioaugmentation richness resident community. These results indicate that is effective remediation soil also provides valuable information about behavior inoculant population during bioremediation, which could be directly used risk assessment optimization process.