Simultaneous Saccharification and Fermentation of Hydrothermal Pretreated Lignocellulosic Biomass: Evaluation of Process Performance Under Multiple Stress Conditions

摘要: Industrial lignocellulosic bioethanol processes are exposed to different environmental stresses (such as inhibitor compounds, high temperature, and solid loadings). In this study, a systematic approach was followed where the liquid fractions were mixed evaluate influence of varied loadings, percentages liquor used fraction determine effect. Ethanol production by simultaneous saccharification fermentation (SSF) hydrothermally pretreated Eucalyptus globulus wood (EGW) studied under combined diverse stress operating conditions (30–38 °C, 60–80 g from hydrothermal treatment or autohydrolysis (containing compounds)/100 ratio between 4 6.4 in SSF/g unwashed EGW) using an industrial Saccharomyces cerevisiae strain supplemented with low-cost byproducts derived agro-food industry. Evaluation these variables revealed that combination temperature higher loadings most significant variable affecting final ethanol concentration cellulose conversion, whereas had impact on productivity. After optimization, 54 g/L (corresponding 85 % conversion 0.51 g/Lh productivity at 96 h) obtained 37 °C 60 16 loading (liquid g/g). The selection suitable along nutritional supplementation enabled produce noticeable titers quite restrictive SSF conditions, which can reduce cost boost economic feasibility lignocellulose-to-ethanol processes.