Characterization of three caspases and their pathogen-induced expression pattern in Portunus trituberculatus.

摘要: Caspases are a family of proteases involved in many important biological processes including apoptosis and inflammation. In this study, we analyzed the expression patterns effects on immune response various tissues edible crab Portunus trituberculatus. PtCas 2, 3 4 share overall sequence identities 55.88%-74.86%, 8.47%-46.54% 20.11%-50.87%, respectively, with their other crustacean species. have same caspase domain catalytic site found known caspases. The levels three caspases differed between tissues. Following bacterial viral infection, reached maximum level at 24 h post-infection (hpi) case bacteria, whereas it was 48 hpi virus. Moreover, WSSV, Vibrio alginolyticus or V. parahaemolyticus induced activities 2-4 time-dependent manner. These results indicate an involvement demonstrate for first time that essential optimal to virus infection crabs.