A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1.
作者： Dujuan Li , Jianping Wang , Ronghui Wang , Yanbin Li , Daad Abi-Ghanem
DOI: 10.1016/J.BIOS.2011.04.010
关键词:
摘要: Abstract As a potential pandemic threat to human health, there has been an urgent need for rapid detection of the highly pathogenic avian influenza (AI) H5N1 virus. In this study, magnetic nanobeads amplification based quartz crystal microbalance (QCM) immunosensor was developed as new method and application AI virus detection. Polyclonal antibodies against surface antigen HA (Hemagglutinin) were immobilized on gold QCM through self-assembled monolayer (SAM) 16-mercaptohexadecanoic acid (MHDA). Target viruses then captured by antibodies, resulting in change frequency. Magnetic (diameter, 30 nm) coated with anti-H5 used further binding reaction between antibody (virus). Both bindings target onto confirmed environmental scanning electron microscopy (ESEM). The could detect at titer higher than 0.0128 unit within 2 h. resulted much better signal lower titers. response antibody–antigen (virus) interaction shown be titer-dependent, linear correlation logarithmic number titers frequency shift found from 0.128 12.8 HA unit. No significant interference observed non-target subtypes such H3N2, H2N2, H4N8. evaluated using chicken tracheal swab samples. This research demonstrated that great alternative rapid, sensitive, specific agricultural, food, clinical
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