Protocols to test the activity of antimicrobial peptides against the honey bee pathogen Paenibacillus larvae.

作者: Jasmin C. Khilnani , Helen J. Wing

DOI: 10.1016/J.MIMET.2015.07.017

关键词:

摘要: Paenibacillus larvae is the causal agent of honey bee disease American Foulbrood. Two enhanced protocols that allow activity antimicrobial peptides to be tested against P. are presented. Proof principle experiments demonstrate peptide defensin 1 active in both assays.

参考文章(21)
P. Casteels, C. Ampe, F. Jacobs, M. Vaeck, P. Tempst, Apidaecins: antibacterial peptides from honeybees. The EMBO Journal. ,vol. 8, pp. 2387- 2391 ,(1989) , 10.1002/J.1460-2075.1989.TB08368.X
Israel Alvarado, Michelle M. Elekonich, Ernesto Abel-Santos, Helen J. Wing, Comparison of in vitro methods for the production of Paenibacillus larvae endospores. Journal of Microbiological Methods. ,vol. 116, pp. 30- 32 ,(2015) , 10.1016/J.MIMET.2015.06.011
Eva Y. Chi, Sampathkumar Krishnan, Theodore W. Randolph, John F. Carpenter, Physical Stability of Proteins in Aqueous Solution: Mechanism and Driving Forces in Nonnative Protein Aggregation Pharmaceutical Research. ,vol. 20, pp. 1325- 1336 ,(2003) , 10.1023/A:1025771421906
H. G. Boman, Antibacterial peptides : basic facts and emerging concepts Journal of Internal Medicine. ,vol. 254, pp. 197- 215 ,(2003) , 10.1046/J.1365-2796.2003.01228.X
I. Alvarado, A. Phui, M. M. Elekonich, E. Abel-Santos, Requirements for In Vitro Germination of Paenibacillus larvae Spores Journal of Bacteriology. ,vol. 195, pp. 1005- 1011 ,(2013) , 10.1128/JB.01958-12
María J. González, Viviana G. Beoletto, Alicia M. Agnese, Marcela C. Audisio, Juan M. Marioli, Purification of Substances from Achyrocline satureioides with Inhibitory Activity Against Paenibacillus larvae, the Causal Agent of American Foulbrood in Honeybees’ Larvae Applied Biochemistry and Biotechnology. ,vol. 175, pp. 3349- 3359 ,(2015) , 10.1007/S12010-015-1506-5
Elke Genersch, American Foulbrood in honeybees and its causative agent, Paenibacillus larvae Journal of Invertebrate Pathology. ,vol. 103, ,(2010) , 10.1016/J.JIP.2009.06.015
R. A. Ilyasov, L. R. Gaifullina, E. S. Saltykova, A. V. Poskryakov, A. G. Nikolaenko, Defensins in the honeybee antiinfectious protection Journal of Evolutionary Biochemistry and Physiology. ,vol. 49, pp. 1- 9 ,(2013) , 10.1134/S0022093013010015
Peter CASTEELS, Christophe AMPE, Lise RIVIERE, Jose DAMME, Christopher ELICONE, Mark FLEMING, Frans JACOBS, Paul TEMPST, Isolation and characterization of abaecin, a major antibacterial response peptide in the honeybee (Apis mellifera). FEBS Journal. ,vol. 187, pp. 381- 386 ,(1990) , 10.1111/J.1432-1033.1990.TB15315.X