Regulation of adenine nucleotide translocase and glycerol 3-phosphate dehydrogenase expression by thyroid hormones in different rat tissues

摘要: Thyroid hormone (T3)-dependent gene expression of the adenine nucleotide translocase (ANT) and FAD-linked glycerol 3-phosphate dehydrogenase (mGPDH) was investigated in several rat tissues. Both proteins provide an important link between cytosolic mitochondrial metabolic pathways seem to be involved stimulation oxygen consumption response T3. Here we show that two ANT isoforms are expressed rat, muscle-specific ANT1 form ubiquitous ANT2 form. The mRNA is not sensitive T3 whereas amount increased 7-9-fold liver heart within 12-48 h after application. Little or no effect on observed kidney brain. changes paralleled by increase protein, thus explaining accelerated ADP/ATP exchange mitochondria isolated from hyperthyroid rats. key role control metabolism evident because mersalyl-sensitive phosphate carrier creatine kinase mRNA, which functionally linked ANT, did respond Similarly exchange, transfer NADH respiratory chain via glycerophosphate shuttle very Recently demonstrated 10-15-fold induction mGPDH administration [Muller Seitz (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 10581-10585]. that, contrast with ANT2, time course fast (4-6 h). Furthermore, induced 6-fold 4-fold kidney. From these results conclude T3-mediated transcriptional leading activity contributes considerably