Fluorometric Studies of Biologically Important Molecular Complexes

摘要: Fluorometry has been extensively employed in studies involving molecular complexes because of its exceptional sensitivity and time resolution. Molecular complexation is widespread nature; indeed, it through that process biopolymers such as DNA enzymes are assembled carry out their functions. Covalent binding holds together strong complexes, while hydrogen-bonding, charge-transfer, electrostatic, hydrophobic interactions mainly responsible for the stability weaker complexes. Some high-molecular-weight contain fluorescent building blocks report on immediate environment; e.g., proteins tryptophan tyrosine amino acid residues. Other biomolecules may be labeled with fluorophores by forming noncovalent or covalent Time-resolved time-integrated fluorometric investigations these systems yield information pertaining to following: (a) the polarity fluidity microenvironment emitter, (b) the occurrence structural fluctuations biomolecule within span light emission, (c) the excited-state time-dependent between emitter solvent neighboring chemical groups biomolecule, (d) the overall shape dimensions biomolecule, (e) and exchange excitation energy other components biomolecule.