Der einfluß von plasmiden unterschiedlicher inkompatibilitätsgruppen auf die serumsensibilität von escherichia coli K12

摘要: Using the method of Henkel and Taylor (11, 18) 60 Escherichia coli K12 J53 strains carrying R-plasmids 15 incompatibility groups (FI, FIme, FII, FIV, FV, FVI, Iα, Iγ Iω, I5, B, C, K, M, S) have been tested with respect to their serumsensitivity in pooled human serum. We found for 41 plasmid a grade resistance serum bactericidal activity 0, 19 1, 2 or 3 according Henkel. Applaying results tests same could all graded category 1. The obtained show that there was not any change sensitivity after uptake plasmid. 29 different plasmids were selected studies low concentration system Survivors' curves 2, 3, 5, 10 20% times incubation (10, 20, 30, 60, 90, 120 180 min) established. In relation control strain E. R− difference percentage survivors 18 caryying M) be demonstrated. However, this only observed concentrations from 1 5% most clearly an time min. into categories dependence strain. pIE 307 403 (Inc FI C) showed strongest increasing resistance. used suitable estimation small alterations serumsensitivity. made us influence other complete O-antigen.