The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples.

摘要: Direct polymerase chain reaction (PCR)-based detection with fecal specimens is hampered by inhibitory compounds, such as bilirubin and bile salts. These compounds showed significant inhibition of PCR at low concentrations (10 to 50 micrograms/ml). For direct analysis, samples must be diluted 500-fold overcome inhibition. Therefore, the magnetic immuno assay (MIPA), which combines immunomagnetic separation using specific monoclonal antibodies PCR, was used directly detect salmonellae in feces from humans. Immunomagnetically extracted stool needed only 10-fold when 1 microgram T4 gene 32 protein added PCR. The MIPA sensitivity obtained 10(5) CFU/ml feces. A panel for Salmonella serogroups E extract clinical samples. occurred 11 out 14 stored 4 degrees C 2 months. a promising, fast method identification.