Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

摘要: The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, acids, and 1,4-naphtoquinones. In this study, the first genetic transformation D. tissues presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 ATCC 15834) were suitable vector organisms in process. Transformation led formation teratoma (transformed shoot) cultures with bacterial rolB gene incorporated into genome single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants characterized by an increased quantity including 1,4-naphtoquinone derivative, plumbagin (up 106.63 mg × g−1 DW), acids (including salicylic, caffeic, ellagic acid), comparison non-transformed plants. Moreover, Rhizobium-mediated highly bactericidal properties teratoma-derived extracts. antibacterial transformed up 33% against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli 7% Pseudomonas aeruginosa. For time, prove possibility transformation. propose may be valuable tool for enhancing secondary metabolite production tissue increase human antibiotic-resistant bacteria. • created Dionaea teratomas. Transformed had synthesis compounds. MBC value was connected acid concentrations.