The effects of farnesoic acid and 20-hydroxyecdysone on vitellogenin gene expression in the lobster, Homarus americanus, and possible roles in the reproductive process.

摘要: Reproduction in female lobster (Homarus americanus) is characterized by the maturation of ovary, with a gradual increase its size as result uptake yolk protein precursor, vitellogenin (Vg) to final product vitellin (Vn). Vn formed aggregation several Vg subunits. In most decapods, hepatopancreas major site biosynthesis. The production controlled endocrine factors. this study, effect farnesoic acid (FA) and 20-hydroxyecdysone (20E) on (HaVg1) was investigated vitro organ explant HaVg1 gene expression stimulated FA or 20E dose-dependent manner. A 2-fold 2.2-fold observed 4.2 microM 0.7 20E, respectively. stimulatory either principally during first 90 min. Stimulation together greater (3.3-fold increase) than that hormone alone. This stimulation also within To study synergistic these two hormones, were tested separately at low concentration (42.3 nM 6.7 nM, respectively). Combined use increased synergistically, but not additively. These findings should contribute our understanding reproduction provide insights into manipulation aquaculture under captive conditions.