Microgravity-induced alterations in signal transduction in cells of the immune system

摘要: Since decades it is known that the activity of cells immune system severely dysregulated in microgravity, however, underlying molecular aspects have not been elucidated yet. The identification gravity-sensitive mechanisms an important and indispensable prerequisite for development counteractive measures to prevent or treat disturbed cell function astronauts during long-term space missions. Moreover, their sensitivity altered gravity renders ideal model understand if how on Earth required normal mammalian signal transduction. We investigated effect simulated weightlessness (2D clinostat) real microgravity (parabolic flights) key pathways a human monocytic T lymphocyte line. found cellular responses strongly depend cell-type conditions which are subjected microgravity. In Jurkat cells, enhanced phosphorylation MAP kinases ERK-1/2, MEK p38 inhibition nuclear translocation NF-kB were predominant weightlessness, either stimulated non-stimulated cells. contrast, U937 responded with overall tyrosine-phosphorylation activation c-jun, whereas PMA-stimulated opposite way reduced compared 1g controls. P53 protein was phosphorylated rapidly gravi-sensitive will only enable us negative effects long time exposure Astronauts, but could also lead novel therapeutic targets general.