Protocadherin-10 acts as a tumor suppressor gene, and is frequently downregulated by promoter methylation in pancreatic cancer cells

摘要: Protocadherin-10 (PCDH10), a member of non-clustered protocadherin family which plays important roles in calcium-dependent cell-cell signal transduction and adhesion. PCDH10 functions as tumor suppressor gene its expression is downregulated by promoter methylation many malignances. In the present study, we explored status, biological effects pancreatic cancer cells, furthermore, mechanism preliminary cells. mRNA level was detected semi-quantitative reverse transcription PCR status examined methylation-specific cells (Capan-1, Panc-1, AsPC-1 BxPC-3) well human normal ductal epithelial HPDE6-C7 used control. The were transfected with plasmid pcDNA3.1-PCDH10 or pcDNA3.1 lipofectamine 2000. function determined CCK-8 assay, colony formation flow cytometry, Transwell invasion assay wound-healing assay. levels apoptosis related proteins western blotting. obviously compared to observed two Capan-1 BxPC-3,and could be restored after treating 5-aza-2'-deoxycytidine trichostatin A cell types. Overexpression can inhibit proliferation, migration, ability induce apoptosis. Ectopic increase PARP, caspase-3, caspase-9 decrease bcl-2, AKT p-AKT. acts gene, frequently down-regulated may via pathway.