Prognostic role of APC and RASSF1A promoter methylation status in cell free circulating DNA of operable gastric cancer patients.

摘要: Gastric carcinogenesis is a multistep process including not only genetic mutations but also epigenetic alterations. The best known and more frequent alteration DNA methylation affecting tumor suppressor genes that may be involved in various carcinogenetic pathways. aim of the present study was to investigate status APC promoter 1A RASSF1A cell free operable gastric cancer patients. Using specific PCR, we examined 73 blood samples obtained from patients with cancer. APC promoters were found methylated 61 (83.6%) 50 (68.5%) examined, none healthy control (p < 0.001). A significant association between lymph node positivity observed = 0.005). Additionally, correlation elevated CEA 0.033) as well CA-19.9 0.032) levels, noticed. Kaplan–Meier estimates survival, significantly favored non-methylated 0.008). No other correlations different variables observed. Serum hypermethylation event early cancer. survival hypermethylated nodal indicative prognostic role for those Additional studies, larger cohort are required further explore whether these findings could serve potential molecular biomarkers and/or response treatments.