Effect of hydrostatic pressure and pH value on the three-dimensional fluorescence spectrum of tyrosine solution with various concentrations of copper ion

摘要: Abstract Three-dimensional excitation–emission matrix (EEM) fluorescence spectroscopy was employed to characterize the effect of hydrostatic pressure on tyrosine (Tyr) solution with various concentrations copper ion (Cu2+) at pH 3.0, 7.4 and 9.0. Based mathematical statistics theory, “mean”, “deviation”, “gravity center” three-dimensional spectra's characteristic parameters were extracted. The spectrum clearly revealed fingerprint-like properties Tyr. results showed that Tyr became weak or even quenched increase in concentration Cu2+ increased changing from 0.1 60 MPa. As increased, quenching stronger. These are consistent those two-dimensional spectrum. This principle this method applicable valid. significance these is overcome overlapping spectra distinguish tryptophan, provide fundamental references when used as a probe explore induced protein structural changes.