Optimization of protein production by Micrococcus luteus for exploring pollutant-degrading uncultured bacteria
作者： Xiaomei Su , Yindong Liu , Jinxing Hu , Linxian Ding , Chaofeng Shen
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摘要: The screening of pollutant-degrading bacteria are limited due to most in the natural environment cannot be cultivated. For purpose resuscitating and stimulating “viable but non-culturable” (VBNC) or uncultured bacteria, Micrococcus luteus proteins more convenient cost-effective than purified resuscitation-promoting factor (Rpf) protein. In this study, medium composition culture conditions were optimized by using statistical experimental design analysis enhance protein production M. luteus. important variables influencing determined Plackett-Burman (PBD) then central composite (CCD) was adopted optimize achieve maximum yield. Results showed that yield 25.13 mg/L (vs. 25.66 predicted) obtained when mineral solution, Lithium L-lactate, initial pH incubation time set at 1.5 ml/L, 8.75 g/L, 7.5 48 h, respectively. predicated values calculated with model very close values. Protein obviously increased optimization fitting well observed fluorescence intensity. These results verified feasibility accuracy strategy. This study provides promising information for exploring highly desirable microorganisms.
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