Culture‐dependent and culture‐independent characterization of potentially functional biphenyl‐degrading bacterial community in response to extracellular organic matter from Micrococcus luteus

作者: Xiao‐Mei Su , Yin‐Dong Liu , Muhammad Zaffar Hashmi , Lin‐Xian Ding , Chao‐Feng Shen

DOI: 10.1111/1751-7915.12266

关键词:

摘要: Biphenyl (BP)-degrading bacteria were identified to degrade various polychlorinated BP (PCB) congers in long-term PCB-contaminated sites. Exploring BP-degrading capability of potentially useful was performed for enhancing PCB bioremediation. In the present study, bacterial composition sediment sample first investigated. Then extracellular organic matter (EOM) from Micrococcus luteus used enhance biodegradation. The effect EOM on community investigated by combining with culture-dependent and culture-independent methods. obtained results indicate that Proteobacteria Actinobacteria predominant sediment. M. luteus could stimulate activity some difficult-to-culture degraders, which contribute significant enhancement response addition mainly Rhodococcus Pseudomonas belonging Gammaproteobacteria respectively. This study provides new insights into exploration functional points out broader BP/PCB degrading, be employed PCB-bioremediation processes.

参考文章(39)
Masao Fukuda, Satoru Shimizu, Noriko Okita, Masashi Seto, Eiji Masai, Structural alteration of linear plasmids encoding the genes for polychlorinated biphenyl degradation in Rhodococcus strain RHA1. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 74, pp. 169- 173 ,(1998) , 10.1023/A:1001732718159
Helena Ćetković, Dubravka Hršak, Nikolina Udiković Kolić, Ana Begonja Kolar, Ines Petrić, Sanja Fingler, Ernest Vončina, Enrichment and characterization of PCB-degrading bacteria as potential seed cultures for bioremediation of contaminated soil Food Technology and Biotechnology. ,vol. 45, pp. 11- 20 ,(2007)
A.S. Kaprelyants, D.B. Kell, Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry Journal of Applied Microbiology. ,vol. 72, pp. 410- 422 ,(1992) , 10.1111/J.1365-2672.1992.TB01854.X
Giulio Zanaroli, Annalisa Balloi, Andrea Negroni, Daniele Daffonchio, Lily Y. Young, Fabio Fava, Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs). Journal of Hazardous Materials. ,vol. 178, pp. 417- 426 ,(2010) , 10.1016/J.JHAZMAT.2010.01.097
Alexandre J. Macedo, Kenneth N. Timmis, Wolf-Rainer Abraham, Widespread capacity to metabolize polychlorinated biphenyls by diverse microbial communities in soils with no significant exposure to PCB contamination. Environmental Microbiology. ,vol. 9, pp. 1890- 1897 ,(2007) , 10.1111/J.1462-2920.2007.01305.X
Meiying Xu, Xingjuan Chen, Mengde Qiu, Xiaowei Zeng, Jian Xu, Daiyong Deng, Guoping Sun, Xiang Li, Jun Guo, Bar-Coded Pyrosequencing Reveals the Responses of PBDE-Degrading Microbial Communities to Electron Donor Amendments PLoS ONE. ,vol. 7, pp. e30439- ,(2012) , 10.1371/JOURNAL.PONE.0030439
N. H. Keep, J. M. Ward, G. Robertson, M. Cohen-Gonsaud, B. Henderson, Bacterial resuscitation factors: revival of viable but non-culturable bacteria. Cellular and Molecular Life Sciences. ,vol. 63, pp. 2555- 2559 ,(2006) , 10.1007/S00018-006-6188-2
Serena Capodicasa, Stefano Fedi, Monica Carnevali, Leonardo Caporali, Carlo Viti, Fabio Fava, Davide Zannoni, Terminal-restriction fragment length polymorphism analysis of biphenyl dioxygenase genes from a polychlorinated biphenyl-polluted soil Research in Microbiology. ,vol. 160, pp. 742- 750 ,(2009) , 10.1016/J.RESMIC.2009.10.001