Kinetic Evidence for a Tetrameric Functional Unit in Hemoglobin

摘要: Abstract The time course of the appearance rapid hemoglobin, as judged by results partial photodissociation carbon monoxide, was followed with a stopped-flow flash photolysis apparatus. Deoxyhemoglobin mixed monoxide and fired after short accurately measured interval. There is marked lag in hemoglobin. Analysis suggests that this not due to first order process following ligand binding. cannot, therefore, be dissociation hemoglobin into subunits or loss 2,3-diphosphoglycerate. appear incompatible schemes having dimer prime unit function. It proposed change reactivity associated occurs at least three molecules have combined. This conclusion supported experiments hybrid species αNOβCO which behaves functional tetramer. significantly influenced