FISH analysis in cell touch preparations and cytological specimens from formalin-fixed fetal autopsies.

摘要: Postmortem studies on still-borns and miscarriages are important to define the sex eventually morphologic anomalies correlated chromosomal aberrations. When conditions for carrying out a cytogenetic study do not exist, these alterations can be investigated by nucleic acid fluorescent in situ hybridization (FISH), which performed interphase nuclei, usually formalin-fixed paraffin embedded tissues or fresh cytological specimens. The objective of present is prove whether this technique successfully applied cell touch preparations specimens obtained from foetal autopsies. was carried 12 abortions some were spontaneous therapeutic. materials formalin-fixed. Cell obtained. FISH using X/Y probes (Vysis) Aneuvysion Kit (05J38-030, Vysis), being chromosomes 13/21 X/Y/18. To verify reliability technique, same reactions also analogous materials. slides evaluable, hybridized nuclei showed distinct signals. All samples adequate analysis without any notable difference results. Moreover, it technically possible perform only but particularly On other hand, use type samples, as compared tissue sections, has advantage presenting intact, noncut with preserved cytomorphology, avoiding problems overlapping making identification real arrangement easier. Diagn. Cytopathol. 2008;36:633–636. © 2008 Wiley-Liss, Inc.