Enhanced neutrophil response in chronic obstructive pulmonary disease

摘要: BACKGROUND—Neutrophils are likely to play a major role in the inflammatory response seen chronic obstructive pulmonary disease (COPD). This study sought address hypothesis that an enhanced neutrophil proinflammatory agents COPD may contribute their recruitment and activation lungs. METHODS—Circulating neutrophils were obtained from 10 patients with COPD, eight long term smokers normal lung function, healthy never smoking controls. The vitro production of reactive oxygen species (ROS) was measured by NADPH oxidase method (respiratory burst) surface expression several adhesion molecules (Mac-1, LFA-1 L-selectin) flow cytometry. Measurements under basal conditions after stimulation phorbol myristate acetate (PMA) tumour necrosis factor alpha (TNFα). mRNA levels p22-phox (a subunit oxidase) Mac-1 (CD11b) also determined reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS—Patients showed respiratory burst compared both (mean (SE) fluorescence intensity (MFI) 15.1 (0.5) v 11.6 (0.5); mean difference -3.4 (95% CI -5.1 -1.8), p<0.01) PMA (MFI 210 (7) 133 (10); -77 -102 -52), p<0.01). 91 (5) 45 (3); -46 -61 -31), p<0.001) TNFα 340 (15) 263 (11); -119 -34), p=0.001). These differences apparent when non-smokers (p<0.05). similar suggesting observed due post-transcriptional regulation. CONCLUSIONS—These results demonstrate which lungs these patients. findings support those other studies have indicated is pathogenesis this disease.