Cloning and characterisation of the rad9 DNA repair gene from Schizosaccharomyces pombe.

摘要: The rad9.192 DNA repair mutant from the fission yeast, Schizosaccharomyces pombe, is sensitive to both UV and ionising radiation. rad9 gene has been cloned by complementation of gamma-ray sensitivity cell line. A 4.3kb HindIII fragment was found confer resistance types region further localised a 2.6kb HindIII-EcoRV fragment, which, sequence analysis, contain sequences capable coding for 427 amino acid protein, if three introns were postulated remove stop codons. confirmed analysis cDNA clones PCR products derived cDNA. product transcription 1.6kb mRNA low abundance. putative protein shows no homology any published sequence. truncated complementing radiation mutant. Deletion not lethal null allele similar phenotype