A simple optical configuration for cell tracking by dark-field microscopy.

作者: Vlatka Antolović , Maja Marinović , Vedrana Filić , Igor Weber

DOI: 10.1016/J.MIMET.2014.06.006

关键词:

摘要: We describe a simple optical configuration for dark-field microscopy at low magnification, realized with the use of standard microscope components. An inherent high contrast makes this method attractive computer-assisted tracking and counting microorganisms. applied setup to measure speed migrating Dictyostelium amoebae.

参考文章(20)
D HADER, M CLAVIEZ, R MERKL, G GERISCH, Responses of Dictyostelium discoideum amoebae to local stimulation by light. Cell Biology International Reports. ,vol. 7, pp. 611- 616 ,(1983) , 10.1016/0309-1651(83)90115-7
Valentin Magidson, Alexey Khodjakov, Circumventing Photodamage in Live-Cell Microscopy Methods in Cell Biology. ,vol. 114, pp. 545- 560 ,(2013) , 10.1016/B978-0-12-407761-4.00023-3
Curt M. Treu, Omar Lupi, Daniel A. Bottino, Eliete Bouskela, Sidestream dark field imaging: the evolution of real-time visualization of cutaneous microcirculation and its potential application in dermatology. Archives of Dermatological Research. ,vol. 303, pp. 69- 78 ,(2011) , 10.1007/S00403-010-1087-7
Amr I. Abdel-Fattah, Mohamed S. El-Genk, Paul W. Reimus, On Visualization of Sub-Micron Particles with Dark-Field Light Microscopy Journal of Colloid and Interface Science. ,vol. 246, pp. 410- 412 ,(2002) , 10.1006/JCIS.2001.7922
Igor Weber, Richard Albrecht, Image processing for combined bright-field and reflection interference contrast video microscopy Computer Methods and Programs in Biomedicine. ,vol. 53, pp. 113- 118 ,(1997) , 10.1016/S0169-2607(97)01810-5
Hiroaki Takagi, Masayuki J. Sato, Toshio Yanagida, Masahiro Ueda, Functional Analysis of Spontaneous Cell Movement under Different Physiological Conditions PLoS ONE. ,vol. 3, pp. e2648- ,(2008) , 10.1371/JOURNAL.PONE.0002648
Houdong Hu, Changbao Ma, Zhaowei Liu, Plasmonic dark field microscopy Applied Physics Letters. ,vol. 96, pp. 113107- ,(2010) , 10.1063/1.3367729
Mark W. Knight, Jonathan Fan, Federico Capasso, Naomi J. Halas, Influence of excitation and collection geometry on the dark field spectra of individual plasmonic nanostructures. Optics Express. ,vol. 18, pp. 2579- 2587 ,(2010) , 10.1364/OE.18.002579
JONATHAN C. HORTON, DAVINA R. HOCKING, DANIEL L. ADAMS, Rapid identification of ocular dominance columns in macaques using cytochrome oxidase, Zif268, and dark-field microscopy. Visual Neuroscience. ,vol. 17, pp. 495- 508 ,(2000) , 10.1017/S0952523800174024
David R. Soll, Computer-assisted three-dimensional reconstruction and motion analysis of living, crawling cells. Computerized Medical Imaging and Graphics. ,vol. 23, pp. 3- 14 ,(1999) , 10.1016/S0895-6111(98)00058-5