Concanavalin A and Other Lectins in the Study of Tumor Cell Surface Organization

摘要: Cell surface structures of two mouse ascites tumors were studied using lectins. The are sublines the spontaneous mammary adenocarcinoma TA3 in strain A, differing main characteristics. Subline TA3-St grows only syngeneic mice and has high expression H-2 antigens. TA3-Ha, contrast, proliferates all strains, low amounts exposed Concanavalin A (Con A), phytohemagglutinin (PHA) Helix pomatia anti hemagglutinin (HP) used agglutination tests, binding experiments with 125I-labelled lectins also fluorescence studies FITC-labelled Con PHA agglutinated cells but not TA3-Ha cells. However, fluorescein-labelled bound to (greater than 90%) both sublines. Moreover cell types contained an identical number receptors. same result was obtained when receptors on compared. HP this case, difference agglutinability between lines due presence or absence All large numbers In contrast majority lacked (See article.) Trypsin released from cells, at time making these agglutinable by PHA. We conclude that Ta3-Ha have a trypsin sensitive glycoprotein (or glycoproteins) detectable HP, which is absent most It possible interferes PHA; whether it responsible for alloantigen remains be seen.