Protein-carbohydrate interaction , : VI. Isolation of concanavalin a by specific adsorption on cross-linked dextran gels

摘要: 1. 1.|A new method is described for the isolation and purification of concanavalin A based on its specific adsorption cross-linked dextran gels subsequent displacement with d-glucose. 2. 2.|The yield by this approx. 2.0–2.4 g/100 g jack bean meal. The chromatographic recovery order 94%. 3. 3.|Concanavalin obtained procedure 98% precipitable NRRL B-1355-S. 4. 4.|(NH4)2SO4 fractionation a saline extract meal showed maximum localization activity to be in fraction precipitated between 0.50 0.60 saturation (NH4)2SO4. 5. 5.|The nature specificity binding have been shown generally similar that A-polysaccharide interaction demonstrating reversal elution protein from Sephadex G-50 same low molecular weight carbohydrates which inhibit interaction.