Mixed culture models for predicting intestinal microbial interactions between Escherichia coli and Lactobacillus in the presence of probiotic Bacillus subtilis

摘要: Bacillus has been proposed as a probiotic due to its in vivo effectiveness the gastrointestinal tract through antimicrobial activities. The present study investigates effects of Lactobacillus alone or presence subtilis MA139 on inhibition pathogenic Escherichia coli K88. Mixed cultures were used predict possible interactions among these bacteria within intestinal animals. B. was first assayed for against E. K88 both under shaking and static culture conditions. A co-culture assay employed conditions test inhibitory reuteri K88, with without addition MA139. results showed that had marked weak well combination spores exerted strong However, by subilis much higher than (P<0.01). significantly decreased pH oxidation-reduction potential values broth compared (P<0.05). viability increased when co-cultured because counts lower role mixed models suggests may produce beneficial increasing lactobacilli subsequently inhibiting growth coli. Therefore, species is recommended.