Culturing Microglia from the Neonatal and Adult Central Nervous System

摘要: Microglia are the resident macrophage-like cells of central nervous system (CNS) and, as such, have critically important roles in physiological and pathological processes such CNS maturation development, multiple sclerosis, spinal cord injury. can be activated recruited to action by neuronal injury or stimulation, axonal damage seen MS ischemic brain trauma resulting from stroke. These immunocompetent members also thought synaptic plasticity under non-pathological conditions. We employ protocols for culturing microglia neonatal adult tissues that aimed maximize viable cell numbers while minimizing confounding variables, presence other types culture debris. utilize large easily discernable components (e.g. cortex, segments), which makes entire process feasible reproducible. The use is a suitable alternative microglia, many pathologies studied mainly affect postnatal cord. systems useful directly testing effect compounds may either inhibit promote microglial activation. Since activation shape outcomes disease CNS, there need vitro cultured studied.