Oligonucleotide-based fluorogenic sensor for simultaneous detection of heavy metal ions
作者： Changlong Hao , Liguang Xua , Changrui Xing , Hua Kuang , Libing Wang
DOI: 10.1016/J.BIOS.2012.04.008
关键词:
摘要: Abstract In this study, we report a new fluorogenic sensor based on fluorescence resonance energy transfer (FRET) for detection of heavy metal ions in aqueous solution. The method showed the advantage being simple, highly sensitive and selective, rapid. donor (CdTe QDs) acceptor (TAMRA or Cy5) are brought into close proximity to one another due Hg 2+ Ag + form strong stable T-Hg -T complexes C-Ag -C complexes, which quenches fluorescent intensity CdTe QDs enables from acceptor. This high sensitivity selectivity when only kind ion (Ag ) exists. Furthermore, assay can also simultaneously detect water media with limit (LOD) 2.5 1.8 nM, separately, satisfactorily meets demands Environmental Protection Agency (EPA) World Health Organization (WHO). exhibits excellent toward . Therefore, is great practical theoretical importance detecting
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