An electrochemiluminescent assay for high sensitive detection of mercury (II) based on isothermal rolling circular amplification
作者： Xiaoming Zhou , Qiang Su , Da Xing
DOI: 10.1016/J.ACA.2011.11.003
关键词:
摘要: Abstract In this study, we firstly demonstrated that Bst DNA polymerase shows specific recognition and function on the T–Hg2+–T biomimetic structure. Based this, a novel available electrochemiluminescence (ECL) sensor for Hg2+ has been developed. strategy, magnet beads tagged primer was designed to complementary region of circular padlock probe but with two T–T mismatches at 3′ end. The mismatched primers cannot be extended by in absence Hg2+. Stable can formed presence Hg2+, thus induces elongation amplification reaction rolling (RCA) mechanism. Subsequently, resulted RCA products are hybridized tris (bipyridine) ruthenium (TBR)-tagged probes detected ECL platform. Current method sub-nanomolar sensitivity excellent selectivity over spectrum interference metal ions.
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