Detection of aneuploidy involving chromosomes 13, 18, or 21, by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes.
作者： H. Tenjin , R. Segraves , D. Pinkel , J. Gray , M. S. Golbus
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摘要: Fluorescence in situ hybridization (FISH) with chromosome-specific probes has been applied to detection of numerical aberrations involving chromosomes 13, 18, and 21 metaphase interphase amniocytes. High-complexity, composite for were used as this study. These constructed libraries Bluescribe plasmids are designated pBS-13, pBS-18, pBS-21. Elements these bind at numerous sites along the target chromosome and, when detected fluorescently, stain essentially entire long arm chromosome. The number (i.e., type which probe was specific) correctly determined 20 samples spreads analyzed 43 nuclei analyzed; all studies conducted blind fashion. results suggest utility FISH rapid amniotic cells.
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