Development of Transgenic Fungi That Kill Human Malaria Parasites in Mosquitoes
作者： W. Fang , J. Vega-Rodriguez , A. K. Ghosh , M. Jacobs-Lorena , A. Kang
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摘要: Metarhizium anisopliae infects mosquitoes through the cuticle and proliferates in hemolymph. To allow M. to combat malaria with advanced infections, we produced recombinant strains expressing molecules that target sporozoites as they travel hemolymph salivary glands. Eleven days after a Plasmodium-infected blood meal, were treated gland midgut peptide 1 (SM1), which blocks attachment of glands; single-chain antibody agglutinates sporozoites; or scorpine, is an antimicrobial toxin. These reduced sporozoite counts by 71%, 85%, 90%, respectively. scorpine [SM1]8:scorpine fusion protein 98%, suggesting Metarhizium-mediated inhibition Plasmodium development could be powerful weapon for combating malaria.
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