Ultraviolet confocal fluorescence microscopy of the in vitro cornea: redox metabolic imaging.

摘要: A laser scanning microscope was fitted with two argon-ion lasers that provided wavelengths in the regions of 364, 488, and 514 nm. Zeiss water objective 25 ×, a numerical aperture 0.8, corrected for UV, used to measure fluorescence from optical sections freshly enucleated rabbit eyes. The confocal both reflected fluorescent modes image situ epithelial endothelial cells. An excitation wavelength 364 nm emission at 400–500 were reduced pyridine nucleotides. We demonstrate feasibility two-dimensional imaging nucleotides corneal