Methods for Measurement of Intermolecular NOEs by Multinuclear NMR Spectroscopy: Application to a Bacteriophage λ N-Peptide/boxB RNA Complex

摘要: New pulse schemes for recording intermolecular NOEs in a molecular complex consisting of 15N,13C labeled and unlabeled components are presented. The sequences select magnetization transferred from protons on the component to proximal molecule. Filtering (suppression signal 13C molecules) is accomplished using adiabatic inversion pulses which swept at rate tuned according one-bond 1H−13C scalar coupling vs carbon chemical shift profile molecule complex. Significantly improved spectra obtained relative data recorded with other purging schemes. Improvements demonstrated experiments where between RNA-unlabeled peptide protein−unlabeled recorded. A discussion structural information amino-terminal arginine rich domain N protein bacteriophage λ boxB RNA new methodology